Studies have revealed that >99% of the methylome within a species is conserved (Li et al., 2015b). It is the remaining 1% which holds the subtle and profound variations within the accessions of same species. The 1% epigenetic variation includes several thousands of differentially methylated regions (DMRs). Mostly, the genes nearby the DMRs do not exhibit any change in gene expression (Eichten et al., 2013, Schmitz et al., 2013a), whereas, ~10-20% of DMRs are negatively associated with gene expression. Subtle variations in the methylated regions are not associated with any significant change in gene expression. Many of the DMRs among the genotypes of a same species are due to misdirected RdDM or altered targeting of heterochromatin (Schmitz et al., 2013a, Schmitz et al., 2013b). Higher levels of DMRs in specific accessions of a species that are associated with functional variation of genes are reckoned to be responsible for maintenance of epigenetic regulation in these accessions (Dubin et al., 2015, Shen et al., 2014). Qualitatively expressed genes (0 or 1 expression) rather than the quantitatively expressed genes have higher chances to be associated with DMRs (Li et al., 2015b). Variations in DNA methylation levels are attributed to epialleles and have been reported to affect traits like, fruit ripening (Manning et al., 2006), floral morphology (Cubas et al., 1999) and anthocyanin component (Chandler, 2007). Quantitative nature of DNA methylation in plants has been further substantiated by analysis of epigenetic recombinant inbred line (epiRIL) populations in Arabidopsis (Johannes et al., 2009, Reinders et al., 2009). EpiRILs can bring out the cryptic information within the genome that has been silenced due to DNA methylation in a portion of the progeny and these variations which otherwise were hidden could be mapped to genomic regions with altered methylation, if accounts for phenotypic variation (Cortijo et al., 2014, Kooke et al., 2015). Efforts to create such epiRILs in crop plants have not been successful because of the lethality of parental DNA methylation mutants with severe effects on crop methylomes (Hu et al., 2014, Li et al., 2014).
Epialleles resulting from epigenetic variations can arise either due to non-genetic or genetic sources (Richards, 2006, Taudt et al., 2016). Non-genetic sources accounts to failure to properly maintain methylation states or off-target sRNA effects. Non-genetic sources involve DNA methylation due to developmental and environmental factors. Genetic sources of epialleles on the other hand can be transposon insertions that changes the chromatin conformation and brings about structural rearrangements (Hollister and Gaut, 2009). Cis or trans arrangements of TE insertions brings changes in methylation through siRNA production via RdDM. Tapping epigenetic variations for crop improvement depends upon the stability of its transmittance. Stable epigenetic inheritance would have high degree of linkage disequilibrium of epialleles with nearby genetic polymorphisms (Springer and Schmitz, 2017). Association mapping or genomic selection studies using high-density single nucleotide polymorphisms (SNP) maps can thus help in mapping DMRs and using it for crop improvement even in the absence of specific causative SNP linked to phenotypic variation (Springer and Schmitz, 2017).
Studies in Arabidopsis have shown that genetic variations like SNPs are more stably inherited than single cytosine methylation across generations. However, DMRs at several hundred of regions (regional methylation) were found to have grater stable inheritance than individual modifications across generations (Becker et al., 2011, Ossowski et al., 2010, Schmitz et al., 2011). Environmental conditions also influence the stability of inheritance of DNA methylation as confirmed by a study in Arabidopsis plants grown for ~100 years in natural environment showed stable methylome inheritance across generations even in varying environment conditions (Hagmann et al., 2015). However, other studies in Arabidopsis and rice showed severe stress may result in unstable methylation inheritance (Jiang et al., 2014, Zheng et al., 2017). DMRs having significant associations with local SNPs or small insertions and deletions (Indels) have been found to have stable inheritance (Schmitz et al., 2013b). In maize, 50% of DMRs were significantly associated with local SNPs suggesting stable epigenetic inheritance (Eichten et al., 2013). These studies also imply that genotypes with greater genetic variations also have more variations in terms of DNA methylation.